MCOLN1 Knockout cell line (T98G)

×

Subscribe Us

By subscription, you consent to allow Ubigene Biosciences to store and process the information provided above to deliver the latest news, research spotlight, and promotions. You can unsubscribe from these communications at anytime.
location: Home > Products > KO Cell Lines > MCOLN1 Knockout cell line (T98G)
All products
  • All products
  • Red Cotton™ gRNA Plasmid Bank
  • KO Cell Lines
  • Wild-type Cell Lines
  • Cas9 Stable Cell Lines
  • Luciferase Stable Cell Lines
  • EGFP Stable Cell Lines
  • Stem Cell Lines
  • Transfection Culture Medium
  • Cell Monoclonal Culture Medium
  • EZ-Stem™ Cell Culture Medium
  • EZ-cryo™ cell freezing medium
  • Kits
  • Lentivirus

MCOLN1 Knockout cell line (T98G) Order

MCOLN1 Knockout cell line (T98G)
Catalog#
YKO-H792
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
MCOLN1 Knockout cell line (T98G)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
MCOLN1 Knockout cell line (T98G)
Morphology
Fibroblast,adherent
Passage ratio
1:2-1:4
Species
Human
Gene Name
MCOLN1
Gene ID
57192
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
88%MEM+10%FBS+1%NEAA+1%Sodium Pyruvate 100 mM Solution
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:T98G
STR Info (Cell bank)
Cell Line:T98G [T98-G]
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 10121012
D13S317 13131313
D7S820 910910
D16S539 13131313
VWA 17201720
TH01 79.379.3
AMEL XYXY
TPOX 8888
CSF1PO 10121012
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the MCOLN1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the T98G cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, MCOLN1 gene knockout MCOLN1 Knockout cell line (T98G) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

Related Popular Products

Wild-type Cell Lines

  • T98G cell line

    Human Glioblastoma Cell Line(T98G)

    Catalog#: YC-D028
    Size: 1*10^6
    Instruction: Human Glioblastoma Cell Line(T98G)

Mouse Genotyping Kit

  • High-performance Mouse Genotyping Kit

    High-performance Mouse Genotyping Kit

    Catalog#: YK-MGH-50;YK-MGH-100;YK-MGH-250
    Size: ~50 tests;~250 tests;~250 tests
    Instruction:

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium

KO Cell Lines

  • MCOLN1 Knockout cell line (Hela)

    MCOLN1 Knockout cell line (Hela)

    Organism: Human
    Gene Name: MCOLN1
    Gene ID: 57192
    Size: 1*10^6
    Catalog#: YKO-H1003
    YKO-H1003(Hela)
  • MCOLN1 Knockout cell line (HCT 116)

    MCOLN1 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: MCOLN1
    Gene ID: 57192
    Size: 1*10^6
    Catalog#: YKO-HS6845
    YKO-HS6845(HCT 116)
  • MCOLN1 Knockout cell line (HEK293)

    MCOLN1 Knockout cell line (HEK293)

    Organism: Human
    Gene Name: MCOLN1
    Gene ID: 57192
    Size: 1*10^6
    Catalog#: YKO-HS5961
    YKO-HS5961(HEK293)

Related Popular Products

Wild-type Cell Lines

Mouse Genotyping Kit

  • High-performance Mouse Genotyping Kit

    High-performance Mouse Genotyping Kit

    Price (USD): -;-;-
    Catalog#: YK-MGH-50;YK-MGH-100;YK-MGH-250
    Size: ~50 tests;~250 tests;~250 tests
    Instruction:

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium

KO Cell Lines

  • MCOLN1 Knockout cell line (Hela)

    MCOLN1 Knockout cell line (Hela)

    Organism: Human
    Gene Name: MCOLN1
    Gene ID: 57192
    Size: 1*10^6
    Catalog#: YKO-H1003
  • MCOLN1 Knockout cell line (HCT 116)

    MCOLN1 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: MCOLN1
    Gene ID: 57192
    Size: 1*10^6
    Catalog#: YKO-HS6845
  • MCOLN1 Knockout cell line (HEK293)

    MCOLN1 Knockout cell line (HEK293)

    Organism: Human
    Gene Name: MCOLN1
    Gene ID: 57192
    Size: 1*10^6
    Catalog#: YKO-HS5961

Contact us